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Validated high-performance liquid chromatographic method for the determination of lamotrigine in human plasma.
- Source :
-
Journal of chromatography. B, Biomedical sciences and applications [J Chromatogr B Biomed Sci Appl] 2000 May 12; Vol. 741 (2), pp. 289-93. - Publication Year :
- 2000
-
Abstract
- A high-performance liquid chromatographic (HPLC) procedure for lamotrigine was developed and validated. Lamotrigine (LTG) and an internal standard were extracted from plasma using liquid-liquid extraction under alkaline conditions into an organic solvent. The method was linear in the range 0.78-46.95 micromol/l, with a mean coefficient of correlation (r)> or =0.99923. The limit of detection (LOD) and limit of quantification (LOQ) were 0.19 and 0.58 micromol/l, respectively. Within- and between-run precision studies demonstrated C.V.<3% at all tested concentrations. LTG median recovery was 86.14%. Antiepileptic drugs tested did not interfere with the assay. The method showed to be appropriate for monitoring LTG in plasma samples.
Details
- Language :
- English
- ISSN :
- 1387-2273
- Volume :
- 741
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Biomedical sciences and applications
- Publication Type :
- Academic Journal
- Accession number :
- 10872598
- Full Text :
- https://doi.org/10.1016/s0378-4347(00)00102-x