Back to Search
Start Over
Expansion of transgenic tobacco protoplasts expressing pumpkin ascorbate oxidase is more rapid than that of wild-type protoplasts.
- Source :
-
Planta [Planta] 2000 May; Vol. 210 (6), pp. 1018-22. - Publication Year :
- 2000
-
Abstract
- When pumpkin (Cucurbita spp., cv. Ebisu Nankin) ascorbate oxidase cDNA was introduced into cultured cells of tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow No. 2) by Agrobacterium-mediated transformation, the transgenic cells expressed and secreted the recombinant pumpkin ascorbate oxidase into the culture medium. These transgenic cells showed no morphological difference from wild-type cells. However, in the presence of applied hormones protoplasts prepared from the transgenic cells elongated more rapidly than those of wild-type cells. We propose that ascorbate oxidase may play a key role in the regulation of cell expansion perhaps by controlling transport processes through the plasma membrane, but not by affecting the cell wall.
- Subjects :
- Cell Division
Cells, Cultured
Chromatography, High Pressure Liquid
Cucurbitaceae genetics
Immunoblotting
Plants, Genetically Modified
Protoplasts cytology
Recombinant Proteins metabolism
Nicotiana cytology
Nicotiana metabolism
Ascorbate Oxidase metabolism
Plants, Toxic
Protoplasts metabolism
Nicotiana genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0935
- Volume :
- 210
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Planta
- Publication Type :
- Academic Journal
- Accession number :
- 10872237
- Full Text :
- https://doi.org/10.1007/s004250050712