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Prolonged perturbations of tumour necrosis factor-alpha and interferon-gamma in mice inoculated with Clostridium piliforme.
- Source :
-
Journal of medical microbiology [J Med Microbiol] 2000 Jun; Vol. 49 (6), pp. 557-563. - Publication Year :
- 2000
-
Abstract
- Clostridium piliforme is an obligate intracellular bacterium that causes enterohepatic disease in many animal species. C. piliforme infections are commonly subclinical in laboratory rats and mice, and little is known about host regulation of disease or of the effects of C. piliforme infections on investigations that use subclinically infected animals. To assess host regulation of subclinical C. piliforme infections and the effects of those infections on laboratory mice, the expression of the pro-inflammatory cytokines tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) was evaluated at 0, 1, 3, 7, 14 and 28 days after inoculation with C. piliforme. Subclinical infection was induced in weanling C. piliforme-susceptible DBA/2 or -resistant C57BL/6 mice with either a toxic or a non-toxic C. piliforme strain. Hepatic lesions and bacteria were demonstrated histologically in both mouse strains for 14 days after inoculation with the toxigenic bacterial strain, but were never demonstrated histologically following inoculation with the non-toxigenic strain. Hepatic TNF-alpha and IFN-gamma mRNA and serum protein levels were similarly elevated in both mouse strains 1 day after inoculation with both C. piliforme strains, as evaluated by reverse transcription PCR and enzyme-linked immunosorbent assays, respectively. Elevation of IFN-gamma persisted for 14 days after inoculation; TNF-alpha remained elevated at 28 days after inoculation.
- Subjects :
- Animals
Clostridium genetics
Clostridium isolation & purification
Clostridium Infections immunology
DNA, Bacterial analysis
Endotoxins analysis
Female
Interferon-gamma genetics
Liver immunology
Liver microbiology
Liver pathology
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
RNA, Messenger analysis
Reverse Transcriptase Polymerase Chain Reaction veterinary
Tumor Necrosis Factor-alpha genetics
Clostridium immunology
Clostridium Infections veterinary
Interferon-gamma biosynthesis
Rodent Diseases immunology
Tumor Necrosis Factor-alpha biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2615
- Volume :
- 49
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 10847210
- Full Text :
- https://doi.org/10.1099/0022-1317-49-6-557