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Higher plant cells: gamma-tubulin and microtubule nucleation in the absence of centrosomes.
- Source :
-
Microscopy research and technique [Microsc Res Tech] 2000 Jun 01; Vol. 49 (5), pp. 487-95. - Publication Year :
- 2000
-
Abstract
- The assembly of the higher plant cytoskeleton poses several fundamental questions. Since different microtubule arrays are successively assembled during the cell cycle in the absence of centrosomes, we can ask how these arrays are assembled and spatially organized. Two hypotheses are under debate. Either multiple nucleation sites are responsible for the assembly and organization of microtubule arrays or microtubule nucleation takes place at one site, the nuclear surface. In the latter case, microtubule nucleation and organization would be two distinct but coregulated processes. During recent years, novel approaches have provided entirely new insights to understand the assembly and dynamics of the plant cytoskeleton. In the present review, we summarize advances made in microscopy and in molecular biology which lead to novel hypotheses and open up new fields of investigation. From the results obtained, it is clear that the higher plant cell is a powerful model system to investigate cytoskeletal organization in acentrosomal eukaryotic cells.<br /> (Copyright 2000 Wiley-Liss, Inc.)
- Subjects :
- Actins physiology
Blotting, Western
Cytoskeleton ultrastructure
Green Fluorescent Proteins
Luminescent Proteins
Microscopy, Confocal
Microtubule-Associated Proteins analysis
Microtubules chemistry
Mutation
Tubulin analysis
Tubulin genetics
Tubulin physiology
Cell Cycle physiology
Cytoskeleton physiology
Microtubules physiology
Plant Physiological Phenomena
Subjects
Details
- Language :
- English
- ISSN :
- 1059-910X
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Microscopy research and technique
- Publication Type :
- Academic Journal
- Accession number :
- 10842376
- Full Text :
- https://doi.org/10.1002/(SICI)1097-0029(20000601)49:5<487::AID-JEMT11>3.0.CO;2-I