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Monoclonal antibody 57B stains tumor tissues that express gene MAGE-A4.
- Source :
-
International journal of cancer [Int J Cancer] 2000 Jun 15; Vol. 86 (6), pp. 835-41. - Publication Year :
- 2000
-
Abstract
- Monoclonal antibody (Mab) 57B, which was raised against a recombinant MAGE-A3 protein, was tested for its ability to stain cells expressing various members of the MAGE-A gene family. COS-7 cells transfected with cDNAs encoding MAGE-A1, A2, A3, A4, A6, or A12 were stained, whereas those transfected with MAGE-A8, A9, A10, or A11 cDNAs were not. However, in tissue sections, we observed a different pattern of staining: the antibody effectively stained the tumors that expressed MAGE-A4 and only these tumors, regardless of the expression of the other MAGE-A genes. It seems, therefore, that at the level of MAGE gene expression found in tumors, a level clearly lower than that observed in transfected COS cells, only the MAGE-A4 protein can be reliably detected. We conclude that the 57B Mab should be useful for tumor diagnosis related to therapeutic vaccination involving MAGE-A4.<br /> (Copyright 2000 Wiley-Liss, Inc.)
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 86
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 10842198
- Full Text :
- https://doi.org/10.1002/(sici)1097-0215(20000615)86:6<835::aid-ijc12>3.0.co;2-n