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Effect of somatostatin on electrogenic ion transport in the duodenum and colon of the mouse, Mus domesticus.
- Source :
-
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2000 Apr; Vol. 125 (4), pp. 459-68. - Publication Year :
- 2000
-
Abstract
- In this study, we have used the mouse intestine and the Ussing short circuit technique to compare the effects and mechanism of action of somatostatin (SST, 0.1 microM) on cAMP- and Ca(2+)-mediated ion secretion in the duodenum and colon of the Swiss-Webster mouse. The cAMP-dependent secretagogues, prostaglandin E(2) (1 microM) and dibutyryl-cAMP (150 microM) increased short circuit current (I(sc)) in both regions, but only the colonic response was inhibited by SST. This inhibition was independent of enteric nerves, suggesting a direct action on the epithelial cells. The Ca(2+)-dependent secretagogue carbachol (10 microM) stimulated a transient increase in I(sc) in both intestinal segments. In the duodenum, SST partially inhibited this increase in I(sc) and both the responses to carbachol and SST were independent of enteric nerves. In the colon, while SST inhibited the carbachol induced increase in I(sc), pre-treatment with tetrodotoxin (750 nM) profoundly inhibited the carbachol induced increase in I(sc), thus markedly reducing the inhibitory effect of SST. This indicates an involvement of the enteric nervous system in the response to carbachol and the action of SST in the colon. These data indicate marked regional differences within the mouse intestine of the effects of SST on ion secretion and demonstrate different mechanisms of action of SST in the duodenum and colon.
- Subjects :
- Animals
Calcium physiology
Carbachol antagonists & inhibitors
Carbachol pharmacology
Colon physiology
Cyclic AMP physiology
Dinoprostone pharmacology
Duodenum metabolism
Duodenum physiology
Enteric Nervous System drug effects
Ion Transport
Male
Membrane Potentials drug effects
Mice
Colon metabolism
Duodenum drug effects
Somatostatin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6433
- Volume :
- 125
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 10840221
- Full Text :
- https://doi.org/10.1016/s1095-6433(00)00170-7