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Cloning and characterization of a myosin from characean alga, the fastest motor protein in the world.
- Source :
-
Journal of biochemistry [J Biochem] 2000 Jun; Vol. 127 (6), pp. 1065-70. - Publication Year :
- 2000
-
Abstract
- In characean algae, very rapid cytoplasmic streaming is generated by sliding movement of an unconventional myosin on fixed actin cables. The speed of this sliding movement is the fastest among many molecular motors known so far. We have cloned a set of overlapping cDNAs encoding the heavy chain of this myosin by immunoscreening with antibody raised against characean myosin. The molecular mass of this heavy chain is 248 kDa, and the protein has a conserved motor domain, six IQ motifs, an extensive alpha-helical coiled-coil domain, and a C-terminal globular domain. Phylogenetic analysis suggested that this myosin belongs to class XI.
- Subjects :
- Amino Acid Motifs
Amino Acid Sequence
Animals
Antibodies, Blocking pharmacology
Blotting, Western
Cloning, Molecular
Escherichia coli genetics
Eukaryota genetics
Molecular Sequence Data
Movement
Myosins genetics
Phylogeny
Protein Structure, Tertiary
Recombinant Proteins chemistry
Sequence Homology, Amino Acid
Eukaryota chemistry
Myosins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0021-924X
- Volume :
- 127
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10833276
- Full Text :
- https://doi.org/10.1093/oxfordjournals.jbchem.a022699