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[Investigation of the protein composition and immunochemical properties of Toxoplasma gondii excretory and secretory antigen].
[Investigation of the protein composition and immunochemical properties of Toxoplasma gondii excretory and secretory antigen].
- Source :
-
Meditsinskaia parazitologiia i parazitarnye bolezni [Med Parazitol (Mosk)] 2000 Jan-Mar (1), pp. 20-4. - Publication Year :
- 2000
-
Abstract
- The major proteins of T. gondii excretory and secretory antigens (ESA) obtained during cultivation of tachyzoites by using cultured Vero cells were shown to have molecular weights of 79, 70, 57, 48, 36, and 29 kD. ESA and somatic antigen immunoblotting demonstrated that there were noticeable differences in the immunoactive proteins of these antigens. The antibodies of the sera of patients with toxoplasmosis mainly interacted with ESA proteins having molecular weights of 79, 70, 57, 48, 36, and 29 kD, 57-kD ESA protein antibodies being present on the immunoblots of all the tested sera. When ESA was used as an antigen during enzyme immunoassay, it showed a high sensitivity in the detection of IgM antigens in congenital toxoplasmosis. At the same time, ESA identified the antibodies of this class in the sera of healthy donors much infrequently than somatic antigen (p < 0.05).
- Subjects :
- Animals
Antigen-Antibody Complex blood
Antigens, Protozoan classification
Case-Control Studies
Chlorocebus aethiops
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G immunology
Molecular Weight
Protozoan Proteins classification
Sensitivity and Specificity
Serologic Tests
Toxoplasmosis immunology
Toxoplasmosis, Congenital immunology
Vero Cells
Antigens, Protozoan analysis
Antigens, Protozoan immunology
Life Cycle Stages
Protozoan Proteins analysis
Protozoan Proteins immunology
Toxoplasma growth & development
Toxoplasma immunology
Subjects
Details
- Language :
- Russian
- ISSN :
- 0025-8326
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Meditsinskaia parazitologiia i parazitarnye bolezni
- Publication Type :
- Academic Journal
- Accession number :
- 10808712