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The genome of Neisseria gonorrhoeae retains the remnants of a two-component regulatory system that once controlled piliation.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2000 May 15; Vol. 186 (2), pp. 197-201. - Publication Year :
- 2000
-
Abstract
- An intact activator-binding site upstream of the sigma(54) promoter of the pilin-encoding pilE gene of Neisseria gonorrhoeae suggests gonococci produce a protein capable of binding this sequence. We cloned a chimeric gene, rsp, that has sequence similarity to both the pilS and pilR genes of Pseudomonas aeruginosa encoding a two-component regulatory system that controls piliation. This gene is transcribed in N. gonorrhoeae and indirect evidence suggests that Rsp binds to the activator-binding site of the pilE gene. Despite this, mutation of rsp has no effect on piliation in N. gonorrhoeae, suggesting that the remnants of this regulatory system have persisted in the genome, despite the loss of its original function.
- Subjects :
- Bacterial Proteins chemistry
DNA-Directed RNA Polymerases genetics
Pili, Sex genetics
Promoter Regions, Genetic
RNA Polymerase Sigma 54
Sigma Factor genetics
Transcription Factors chemistry
Transcription Factors genetics
Bacterial Proteins genetics
DNA-Binding Proteins
Fimbriae Proteins
Genome, Bacterial
Membrane Glycoproteins genetics
Neisseria gonorrhoeae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 186
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 10802171
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2000.tb09104.x