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Functional analysis of an eye enhancer of the Drosophila eyes absent gene: differential regulation by eye specification genes.
- Source :
-
Developmental biology [Dev Biol] 2000 May 15; Vol. 221 (2), pp. 355-64. - Publication Year :
- 2000
-
Abstract
- Genes involved in eye development are highly conserved between vertebrates and Drosophila. Given the complex genetic network controlling early eye development, identification of regulatory sequences controlling gene expression will provide valuable insights toward understanding central events of early eye specification. We have focused on defining regulatory elements critical for Drosophila eyes absent (eya) expression. Although eya has a complex expression pattern during development, analysis of eye-specific mutations in the gene revealed a region selectively deleted in the eye-specific alleles. Here we have performed detailed analysis of the region deleted in the eye-specific eya(2) allele. This analysis shows that this region can direct early eya gene expression in a pattern consistent with that of normal eya in eye progenitor cells. Functional studies indicate that this element will restore appropriate eya transcript expression to rescue the eye-specific allele. We have examined regulation of this element during eye specification, both in normal eye development and in ectopic eye formation. These studies demonstrate that the element was activated upon ectopic expression of the eye specification genes eyeless and dachshund, but does not respond to ectopic expression of eya or sine oculis. The differential regulation of this element by genes involved during early retinal formation reveals new aspects of the genetic hierarchy of eye development.<br /> (Copyright 2000 Academic Press.)
- Subjects :
- Animals
Animals, Genetically Modified
Crosses, Genetic
DNA-Binding Proteins genetics
Eye transplantation
Genes, Insect
Mutagenesis
Nuclear Proteins genetics
Regulatory Sequences, Nucleic Acid
Sequence Deletion
Drosophila embryology
Drosophila genetics
Drosophila Proteins
Enhancer Elements, Genetic
Eye embryology
Eye Proteins genetics
Gene Expression Regulation, Developmental
Subjects
Details
- Language :
- English
- ISSN :
- 0012-1606
- Volume :
- 221
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 10790331
- Full Text :
- https://doi.org/10.1006/dbio.2000.9688