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New world monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution.
- Source :
-
Journal of biochemistry [J Biochem] 2000 May; Vol. 127 (5), pp. 761-70. - Publication Year :
- 2000
-
Abstract
- Pepsinogens A and C, and prochymosin were purified from four species of adult New World monkeys, namely, common marmoset (Callithrix jacchus), cotton-top tamarin (Saguinus oedipus), squirrel monkey (Saimiri sciureus), and capuchin monkey (Cebus apella). The occurrence of prochymosin was quite unique since this zymogen is known to be neonate-specific and, in primates, it has been thought that the prochymosin gene is not functional. No multiple form has been detected for any type of pepsinogen except that two pepsinogen-A isozymogens were identified in capuchin monkey. Pepsins A and C, and chymosin hydrolyzed hemoglobin optimally at pH 2-2.5 with maximal activities of about 20, 30, and 15 units/mg protein. Pepsins A were inhibited in the presence of an equimolar amount of pepstatin, and chymosins and pepsins C needed 5- and 100-fold molar excesses of pepstatin for complete inhibition, respectively. Hydrolysis of insulin B chain occurred first at the Leu15-Tyr16 bond in the case of pepsins A and chymosins, and at either the Leu15-Tyr16 or Tyr16-Leu17 bond in the case of pepsins C. The presence of different types of pepsins might be advantageous to New World monkeys for the efficient digestion of a variety of foods. Molecular cloning of cDNAs for three types of pepsinogens from common marmoset was achieved. A phylogenetic tree of pepsinogens based on the nucleotide sequence showed that common marmoset diverged from the ancestral primate about 40 million years ago.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Chymosin antagonists & inhibitors
Cloning, Molecular
DNA, Complementary genetics
Enzyme Precursors antagonists & inhibitors
Likelihood Functions
Molecular Sequence Data
Pepsinogen A antagonists & inhibitors
Pepsinogen A chemistry
Pepsinogen C antagonists & inhibitors
Pepsinogen C chemistry
Sequence Homology, Amino Acid
Substrate Specificity
Vertebrates genetics
Cebidae genetics
Chymosin genetics
Enzyme Precursors genetics
Evolution, Molecular
Pepsinogen A genetics
Pepsinogen C genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-924X
- Volume :
- 127
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10788784
- Full Text :
- https://doi.org/10.1093/oxfordjournals.jbchem.a022668