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Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebrafish (Danio rerio).
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 2000 Jan 31; Vol. 1490 (1-2), pp. 175-83. - Publication Year :
- 2000
-
Abstract
- We have cloned a cDNA from zebrafish (Danio rerio) that contains an open-reading frame of 132 amino acids coding for a fatty acid binding protein (FABP) of approximately 15 kDa. Multiple sequence alignment revealed extensive amino acid identity between this zebrafish FABP and intestinal-like FABPs (I-FABP) from other species. The zebrafish I-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases PstI Bg/II or EcoRI suggesting that a single copy of the I-FABP gene is present in the zebrafish genome. An oligonucleotide probe complementary to the zebrafish I-FABP mRNA hybridized to an mRNA of approximately 800 bases in Northern blot analysis. In situ hybridization revealed that the I-FABP mRNA was expressed exclusively in the intestine of the adult zebrafish.
- Subjects :
- Amino Acid Sequence
Animals
Autoradiography
Base Sequence
Carrier Proteins metabolism
Cloning, Molecular
Fatty Acid-Binding Protein 7
Fatty Acid-Binding Proteins
Female
Gene Expression Regulation
In Situ Hybridization
Intestinal Mucosa metabolism
Male
Microvilli metabolism
Molecular Sequence Data
Myelin P2 Protein metabolism
RNA, Messenger analysis
Sequence Alignment
Zebrafish metabolism
Carrier Proteins genetics
Myelin P2 Protein genetics
Neoplasm Proteins
Nerve Tissue Proteins
Zebrafish genetics
Zebrafish Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1490
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 10786634
- Full Text :
- https://doi.org/10.1016/s0167-4781(99)00229-8