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FabG, an NADPH-dependent 3-ketoacyl reductase of Pseudomonas aeruginosa, provides precursors for medium-chain-length poly-3-hydroxyalkanoate biosynthesis in Escherichia coli.
- Source :
-
Journal of bacteriology [J Bacteriol] 2000 May; Vol. 182 (10), pp. 2978-81. - Publication Year :
- 2000
-
Abstract
- Escherichia coli hosts expressing fabG of Pseudomonas aeruginosa showed 3-ketoacyl coenzyme A (CoA) reductase activity toward R-3-hydroxyoctanoyl-CoA. Furthermore, E. coli recombinants carrying the poly-3-hydroxyalkanoate (PHA) polymerase-encoding gene phaC in addition to fabG accumulated medium-chain-length PHAs (mcl-PHAs) from alkanoates. When E. coli fadB or fadA mutants, which are deficient in steps downstream or upstream of the 3-ketoacyl-CoA formation step during beta-oxidation, respectively, were transformed with fabG, higher levels of PHA were synthesized in E. coli fadA, whereas similar levels of PHA were found in E. coli fadB, compared with those of the corresponding mutants carrying phaC alone. These results strongly suggest that FabG of P. aeruginosa is able to reduce mcl-3-ketoacyl-CoAs generated by the beta-oxidation to 3-hydroxyacyl-CoAs to provide precursors for the PHA polymerase.
- Subjects :
- 3-Hydroxyacyl CoA Dehydrogenases genetics
Acyltransferases genetics
Alcohol Oxidoreductases genetics
Cloning, Molecular
Escherichia coli genetics
Genes, Bacterial
Hydroxy Acids metabolism
Recombination, Genetic
3-Hydroxyacyl CoA Dehydrogenases metabolism
Escherichia coli metabolism
Pseudomonas aeruginosa enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 182
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 10781572
- Full Text :
- https://doi.org/10.1128/JB.182.10.2978-2981.2000