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Cellular repair mechanism of 5-formyluracil.
- Source :
-
Nucleic acids symposium series [Nucleic Acids Symp Ser] 1999 (42), pp. 291-2. - Publication Year :
- 1999
-
Abstract
- 5-Formyluracil (fU) is an oxidative DNA base damage. This damage has been suggested to be mutagenic and but enzymatic repair of the damage is little known. In this study, repair enzymes that recognize fU have been studied. Kinetic analysis of the repair activity of E. coli 3-methyladenine DNA glycosylase II (AlkA) showed that fU was removed by AlkA with the efficiency comparable to 7-methylguanine. We also examined the participation of the methyl-directed mismatch repair system. The affinity of MutS to the fU:G mispair was essentially similar to that of the T:G mispair that was most efficiently recognized by the MutSLH system. These results suggest two distinct repair pathways of fU in E. coli.
- Subjects :
- Base Pair Mismatch
Cloning, Molecular
DNA Damage
DNA-(Apurinic or Apyrimidinic Site) Lyase
Deoxyribonuclease IV (Phage T4-Induced)
Recombinant Proteins metabolism
Carbon-Oxygen Lyases metabolism
DNA Glycosylases
DNA Repair
Escherichia coli enzymology
Escherichia coli Proteins
N-Glycosyl Hydrolases metabolism
Uracil analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0261-3166
- Issue :
- 42
- Database :
- MEDLINE
- Journal :
- Nucleic acids symposium series
- Publication Type :
- Academic Journal
- Accession number :
- 10780494
- Full Text :
- https://doi.org/10.1093/nass/42.1.291