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Activation of transcription factors NF-kappaB and NF-IL-6 by human immunodeficiency virus type 1 protein R (Vpr) induces interleukin-8 expression.
- Source :
-
Journal of virology [J Virol] 2000 May; Vol. 74 (10), pp. 4658-65. - Publication Year :
- 2000
-
Abstract
- Human immunodeficiency virus (HIV)-positive individuals express elevated levels of interleukin-8 (IL-8), which is believed to be responsible for some of the clinical manifestations occurring during AIDS. We report here that virion-derived HIV type 1 (HIV-1) protein R (Vpr) increased IL-8 expression in primary T cells and macrophages, as well as in the T-cell line Jurkat, the monocytic cell line U937, and the epithelial cell line A549. Vpr appeared to increase IL-8 expression and IL-8 promoter activity by activating transcription factors NF-kappaB and NF-IL-6. Elevated Vpr was also shown to increase transcription of the NF-kappaB and NF-IL-6 enhancer-containing viral promoters for HIV, cytomegalovirus, and simian virus 40, as well as increase the expression of IL-6 and IL-10 in primary macrophages and in A549 cells, tumor necrosis factor alpha expression in primary T cells, and IL-6 and gamma interferon expression in U937 cells. These results suggest a new role for Vpr in the pathogenesis of HIV infection, namely, the activation of transcription factors NF-IL-6 and NF-kappaB.
- Subjects :
- CCAAT-Enhancer-Binding Proteins
Cell Line
Cells, Cultured
Cytokines biosynthesis
Gene Products, vpr genetics
HIV-1 genetics
Humans
Interleukin-8 genetics
Jurkat Cells
Macrophages metabolism
Plasmids
Promoter Regions, Genetic
T-Lymphocytes metabolism
vpr Gene Products, Human Immunodeficiency Virus
DNA-Binding Proteins metabolism
Gene Products, vpr metabolism
HIV-1 metabolism
Interleukin-8 biosynthesis
NF-kappa B metabolism
Nuclear Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 74
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 10775602
- Full Text :
- https://doi.org/10.1128/jvi.74.10.4658-4665.2000