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Angiotensin I-converting enzyme activity and vascular sensitivity to angiotensin I in rat injured carotid artery.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2000 Apr 14; Vol. 394 (2-3), pp. 301-9. - Publication Year :
- 2000
-
Abstract
- We used a vasoreactivity assay to examine the functional significance of angiotensin I-converting enzyme overexpression in smooth muscle cells after vascular injury. Rat carotid arteries isolated at days 2 to 14 after in vivo endothelial denudation were compared with the contralateral freshly denuded (control) vessels. Arterial rings were constricted ex vivo with angiotensin I in the absence or presence of the angiotensin I-converting enzyme inhibitors captopril (300 nM and 3 microM) or perindoprilate (1 nM). Angiotensin I-converting enzyme activity was determined by cleavage of the chromogenic substrate Hip-His-Leu. Angiotensin I-converting enzyme activity in injured arteries was increased (2-fold) at day 7 only after vascular injury. Contractions to angiotensin I were unaffected after injury. Inhibition by captopril and perindoprilate of angiotensin I-induced contractions was significantly less potent in injured arteries at day 7 as compared to control vessels. Mechanical removal of neointimal smooth muscle cells normalized the inhibition by captopril in injured arteries at day 7. Captopril did not affect angiotensin II-induced contractions. Thus, upregulation of angiotensin I-converting enzyme after arterial injury confers resistance to angiotensin I-converting enzyme inhibitors.
- Subjects :
- Angioplasty, Balloon
Animals
Carotid Artery Injuries pathology
Endothelium, Vascular drug effects
Endothelium, Vascular physiology
Male
Muscle Contraction drug effects
Phenylephrine pharmacology
Potassium Chloride pharmacology
Rats
Rats, Sprague-Dawley
Time Factors
Up-Regulation drug effects
Vasoconstrictor Agents pharmacology
Angiotensin I pharmacology
Carotid Artery Injuries drug therapy
Muscle, Smooth, Vascular drug effects
Peptidyl-Dipeptidase A metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2999
- Volume :
- 394
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 10771296
- Full Text :
- https://doi.org/10.1016/s0014-2999(00)00071-6