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[Synthesis and immunochemical properties of the recombinant major surface glycoprotein E2 of the classical swine fever virus].
- Source :
-
Voprosy virusologii [Vopr Virusol] 2000 Mar-Apr; Vol. 45 (2), pp. 29-36. - Publication Year :
- 2000
-
Abstract
- Recombinant E2 protein from vaccine strain of classical swine fever virus (CSFV) and from SCFV virulent strain Shimen was synthesized in SF-21 and High-Five cell culture with baculovirus as the expressing vector. For secretion, hydrophobic C-terminal transmembrane domain was removed and N-terminal signal polypeptide of 38 amino acids was added. Maximum accumulation of recombinant products in SF-21 cells was observed after 48 h and in medium 96 h after infection with recombinant baculovirus. In High-Five cells and in culture medium the maximum accumulation of E2 was observed after 96 h. The level of E2 expression is 5-10 micrograms/106 cells. The products of expression were purified by affinity chromatography and their specificity confirmed in immunochemical tests with a series of reference monoclonal antibodies. The product can be used for detecting antibodies to SCFV by competitive enzyme immunoassay.
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Baculoviridae genetics
Base Sequence
Cell Line
Chromatography, Affinity
DNA Primers
Immunoenzyme Techniques
Recombinant Proteins genetics
Recombinant Proteins immunology
Recombinant Proteins metabolism
Spodoptera
Viral Envelope Proteins immunology
Viral Envelope Proteins metabolism
Viral Envelope Proteins genetics
Subjects
Details
- Language :
- Russian
- ISSN :
- 0507-4088
- Volume :
- 45
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Voprosy virusologii
- Publication Type :
- Academic Journal
- Accession number :
- 10765548