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[Spectrophotometric and fluorometric study of albumins and hemin interactions with aromatic antioxidants].
- Source :
-
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2000 Jan-Feb; Vol. 36 (1), pp. 44-54. - Publication Year :
- 2000
-
Abstract
- Difference spectrophotometry and fluorescence quenching of human and bovine serum albumins were used to determine their association constants (Ka) with hemin in buffered physiological saline (pH 7.4) supplemented with 2% dimethylsulfoxide or in 40% aqueous dimethylformamide (pH 7.4). Ka values depended on the medium, the extent of albumin delipidation, and on the method of determination. The formation of hemin complexes with o-phenylenediamine, tetramethylbenzidine, gallic acid, its polydisulfide, and two substituted di-tert-butyl pyrocatechols was studied by difference spectrophotometry in the same media; Ka values for the complexes were calculated and compared to each other. The formation of complexes of these aromatic ligands with albumins was studied fluorometrically; Ka values were of order of approximately 10(-5) M-1 and decreased with the ligand hydrophobicity.
Details
- Language :
- Russian
- ISSN :
- 0555-1099
- Volume :
- 36
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Prikladnaia biokhimiia i mikrobiologiia
- Publication Type :
- Academic Journal
- Accession number :
- 10752084