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gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes.
- Source :
-
The EMBO journal [EMBO J] 2000 Apr 03; Vol. 19 (7), pp. 1458-66. - Publication Year :
- 2000
-
Abstract
- InlB is a Listeria monocytogenes protein that promotes entry of the bacterium into mammalian cells by stimulating tyrosine phosphorylation of the adaptor proteins Gab1, Cbl and Shc, and activation of phosphatidyl- inositol (PI) 3-kinase. Using affinity chromatography and enzyme-linked immunosorbent assay, we demonstrate a direct interaction between InlB and the mammalian protein gC1q-R, the receptor of the globular part of the complement component C1q. Soluble C1q or anti-gC1q-R antibodies impair InlB-mediated entry. Transient transfection of GPC16 cells, which are non-permissive to InlB-mediated entry, with a plasmid-expressing human gC1q-R promotes entry of InlB-coated beads. Furthermore, several experiments indicate that membrane recruitment and activation of PI 3-kinase involve an InlB-gC1q-R interaction and that gC1q-R associates with Gab1 upon stimulation of Vero cells with InlB. Thus, gC1q-R constitutes a cellular receptor involved in InlB-mediated activation of PI 3-kinase and tyrosine phosphorylation of the adaptor protein Gab1. After E-cadherin, the receptor for internalin, gC1q-R is the second identified mammalian receptor promoting entry of L. monocytogenes into mammalian cells.
- Subjects :
- Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Antibodies pharmacology
Bacterial Proteins genetics
Base Sequence
Binding, Competitive
Carrier Proteins
Cell Line
Chlorocebus aethiops
DNA Primers genetics
HeLa Cells
Humans
Listeria monocytogenes genetics
Listeria monocytogenes pathogenicity
Membrane Proteins genetics
Mitochondrial Proteins
Molecular Sequence Data
Phosphoproteins metabolism
Phosphorylation
Receptors, Complement antagonists & inhibitors
Receptors, Complement genetics
Transfection
Tyrosine metabolism
Vero Cells
Bacterial Proteins metabolism
Complement C1q metabolism
Hyaluronan Receptors
Listeria monocytogenes metabolism
Membrane Glycoproteins
Membrane Proteins metabolism
Receptors, Complement metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0261-4189
- Volume :
- 19
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The EMBO journal
- Publication Type :
- Academic Journal
- Accession number :
- 10747014
- Full Text :
- https://doi.org/10.1093/emboj/19.7.1458