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Psoriasis patients with basal cell carcinoma have more repair-mediated DNA strand-breaks after UVC damage in lymphocytes than psoriasis patients without basal cell carcinoma.
- Source :
-
Cancer letters [Cancer Lett] 2000 Apr 14; Vol. 151 (2), pp. 187-92. - Publication Year :
- 2000
-
Abstract
- We have investigated the formation of strand-breaks following UVC irradiation in lymphocytes from psoriasis patients with or without basal cell carcinoma (BCC). Isolated lymphocytes were irradiated with UVC light at a dose of 3.6 J/m(2), and the level of DNA strand-breaks were measured 25 min after the irradiation by the alkaline comet assay. The generation of strand-breaks following UVC irradiation indicates DNA-repair-mediated incisions, as UVC light does not generate strand-breaks per se. We found that psoriasis patients with BCC had more DNA-repair incisions than non-cancer patients. The incision level correlated to two polymorphisms of the XPD gene. At present, it is not clear if the association is a primary effect that is related to differences of the XPD protein. Genes encoding for other repair proteins, namely XRCC1, ERCC1, and LIG1 are located close to the XPD gene, and it is possible that the association is due to a cosegregation with a polymorphism in one of these genes.
- Subjects :
- Alleles
Carcinoma, Basal Cell pathology
Cells, Cultured
Comet Assay
DNA Damage genetics
DNA Repair radiation effects
Dose-Response Relationship, Radiation
Exons genetics
Female
Genetic Linkage genetics
Genetic Predisposition to Disease genetics
Humans
Lymphocytes metabolism
Lymphocytes pathology
Male
Polymorphism, Genetic genetics
Proteins genetics
Psoriasis pathology
Skin Neoplasms complications
Skin Neoplasms pathology
Ultraviolet Rays
Xeroderma Pigmentosum Group D Protein
Carcinoma, Basal Cell complications
Carcinoma, Basal Cell genetics
DNA Damage radiation effects
DNA Helicases
DNA Repair genetics
DNA-Binding Proteins
Lymphocytes radiation effects
Psoriasis complications
Psoriasis genetics
Skin Neoplasms genetics
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3835
- Volume :
- 151
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cancer letters
- Publication Type :
- Academic Journal
- Accession number :
- 10738113
- Full Text :
- https://doi.org/10.1016/s0304-3835(99)00414-0