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A sleep inducing factor from common Indian toad (Bufo melanostictus, Schneider) skin extract.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 2000 Sep; Vol. 38 (9), pp. 1267-81. - Publication Year :
- 2000
-
Abstract
- Bufo melanostictus (common Indian toad) acquire different bioactive substances in their skin during their life-time in wide ecological habitat. Earlier investigation from this laboratory revealed that toad (B. melanostictus) skin extract (TSE) posses different bioactive compounds of different diversity (Das, M., Auddy, B. and Gomes, A., 1996. Pharmacological study of the toad skin extract on experimental animals. Indian J. Pharmacol. 28, 72-76). Among these sleep induction and sleep potentiation indicated the possibility of sleep inducing factor(s) in TSE. One such sleep inducing factor (SIF) was isolated and purified by neutral alumina column chromatography followed by HPLC. Spectroscopy (UV, IR, FAB-MASS) study indicated that the sleep inducing factor was a 880 Dalton conjugated aromatic compound with a hydroxyl and carbonyl functional group. Biological study showed that SIF produced no lethality in male albino mice upto the dose of 8 mg/kg, i.v. Cyproheptadine antagonised SIF induced contraction of isolated smooth muscle indicating histamine/serotonin receptor mediated action of SIF. EEG studies showed that SIF increased sleep and decreased awakening condition of freely moving rats. Biochemical studies showed that SIF produced significant alteration of brain biogenic amine levels, monoamine oxidase (MAO) and tryptophan hydroxylase (TH) activity. This may be the reason of SIF induced sleep, although the SIF induced sleep mechanism needs further detail investigation.
- Subjects :
- Animals
Biogenic Amines metabolism
Brain Chemistry drug effects
Bufonidae
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Guinea Pigs
In Vitro Techniques
Lethal Dose 50
Male
Mice
Monoamine Oxidase metabolism
Muscle, Smooth drug effects
Polysomnography drug effects
Postural Balance drug effects
Rats
Skin chemistry
Spectrophotometry, Ultraviolet
Tissue Extracts toxicity
Tryptophan Hydroxylase metabolism
Skin Physiological Phenomena
Sleep drug effects
Tissue Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0041-0101
- Volume :
- 38
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 10736480
- Full Text :
- https://doi.org/10.1016/s0041-0101(99)00229-9