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Rat receptor-activity-modifying proteins (RAMPs) for adrenomedullin/CGRP receptor: cloning and upregulation in obstructive nephropathy.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2000 Apr 02; Vol. 270 (1), pp. 89-93. - Publication Year :
- 2000
-
Abstract
- Adrenomedullin (AM) is a potent vasorelaxing peptide originally isolated pheochromocytoma. Recently, a family of receptor-activity-modifying proteins (RAMPs 1-3) were identified in humans. Associated with the calcitonin receptor-like receptor (CRLR), RAMP2 or RAMP3 may function as the AM receptor. Here we cloned rat RAMP family, analyzed their distribution in rat tissues, and examined regulation of their expression in the kidney using an obstructive nephropathy model. Northern blot analyses revealed that the RAMP family genes are expressed in various tissues with different tissue specificity; RAMP1 is abundantly expressed in the brain, fat, thymus, and spleen, RAMP2 in the lung, spleen, fat, and aorta, while RAMP3 is most abundant in the kidney and lung. After ureteral obstruction, RAMP1, RAMP2, and CRLR gene expressions in the obstructed kidney were markedly upregulated, whereas RAMP3 expression was unchanged. Thus, RAMPs are regulated differently in obstructive nephropathy, suggesting their distinct roles in renal pathophysiology.<br /> (Copyright 2000 Academic Press.)
- Subjects :
- Amino Acid Sequence
Animals
Cloning, Molecular
Fibrosis etiology
Fibrosis metabolism
Intracellular Signaling Peptides and Proteins
Kidney Diseases etiology
Male
Molecular Sequence Data
Rats
Rats, Wistar
Receptor Activity-Modifying Protein 1
Receptor Activity-Modifying Protein 2
Receptor Activity-Modifying Protein 3
Receptor Activity-Modifying Proteins
Receptors, Adrenomedullin
Sequence Homology, Amino Acid
Up-Regulation
Kidney Diseases metabolism
Membrane Proteins genetics
Receptors, Calcitonin Gene-Related Peptide
Receptors, Peptide
Ureteral Obstruction complications
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 270
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 10733909
- Full Text :
- https://doi.org/10.1006/bbrc.2000.2390