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Fusion of the green fluorescent protein to amino acids 1 to 71 of bovine respiratory syncytial virus glycoprotein G directs the hybrid polypeptide as a class II membrane protein into the envelope of recombinant bovine herpesvirus-1.
- Source :
-
The Journal of general virology [J Gen Virol] 2000 Apr; Vol. 81 (Pt 4), pp. 1051-5. - Publication Year :
- 2000
-
Abstract
- It was recently shown that the class II membrane glycoprotein G of bovine respiratory syncytial virus (BRSV) is integrated into the envelope of recombinant bovine herpesvirus-1 (BHV-1) virions in the correct orientation. To verify the hypothesis that the membrane anchor of BRSV G might be suitable to target heterologous polypeptides into the membrane of recombinant BHV-1 particles, an open reading frame encoding a fusion protein between amino acids 1 to 71 of the BRSV G glycoprotein and the green fluorescent protein (TMIIGFP) was recombined into the genome of BHV-1. The resulting recombinant BHV-1/eTMIIGFP had growth properties similar to those of wild-type BHV-1. Live-cell analysis of cells infected with BHV-1/eTMIIGFP indicated that the fusion protein localized to the cell surface. Immunoprecipitations and virus neutralization assays using a GFP-specific antiserum proved that TMIIGFP was incorporated as a class II membrane protein into virions.
- Subjects :
- Animals
Cattle
Green Fluorescent Proteins
Herpesvirus 1, Bovine metabolism
Luminescent Proteins metabolism
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Recombination, Genetic
Respiratory Syncytial Virus, Bovine metabolism
Viral Envelope Proteins metabolism
Herpesvirus 1, Bovine genetics
Luminescent Proteins genetics
Respiratory Syncytial Virus, Bovine genetics
Viral Envelope Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 81
- Issue :
- Pt 4
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 10725432
- Full Text :
- https://doi.org/10.1099/0022-1317-81-4-1051