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Molecular and immunological analyses of the Borrelia turicatae Bdr protein family.
- Source :
-
Infection and immunity [Infect Immun] 2000 Apr; Vol. 68 (4), pp. 2369-73. - Publication Year :
- 2000
-
Abstract
- Here, we describe the molecular and immunological characterization of the bdr gene family of Borrelia turicatae, a relapsing-fever spirochete. Nine bdr alleles belonging to two different subfamilies were sequenced and localized to linear plasmids. Anti-Bdr antiserum was generated and used to analyze Bdr expression in pre- and postinfection isogenic populations. The analyses presented here provide a detailed characterization of the Bdr proteins in a relapsing-fever spirochete species, enhancing our understanding of these proteins at the genus-wide level.
- Subjects :
- Alleles
Amino Acid Sequence
Animals
Escherichia coli metabolism
Evolution, Molecular
Gene Library
Immunoblotting
Male
Mice
Mice, Inbred C3H
Molecular Sequence Data
Multigene Family
Oligonucleotide Probes
Open Reading Frames
Plasmids
Sequence Homology, Amino Acid
Temperature
Bacterial Proteins chemistry
Borrelia genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 68
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 10722647
- Full Text :
- https://doi.org/10.1128/IAI.68.4.2369-2373.2000