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Fluid shear stress induces heat shock protein 60 expression in endothelial cells in vitro and in vivo.
- Source :
-
Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2000 Mar; Vol. 20 (3), pp. 617-23. - Publication Year :
- 2000
-
Abstract
- Recent investigations indicate that the initial event in the pathogenesis of atherosclerosis involves an (auto)immunologic injury to the vessel wall. Heat shock proteins (hsps), which are expressed on the endothelial cell surface, constitute possible autoantigens. After being exposed to shear stress of 30 dyne/cm(2) in vitro by means of a rotational viscometer, human umbilical vein endothelial cells were immunohistochemically stained for hsp 60 by the monoclonal antibody ML-30; static control cells were negative. Maximal hsp 60 induction was observed after 12 hours of hemodynamic stress. In Northern blots, the level of hsp 60 mRNA was markedly increased after only 1 hour of shear stress in human umbilical vein endothelial cells compared with static control cells. In vivo investigations in Lewis rats confirmed these in vitro findings: the intima and media of frozen sections of the right common carotid artery exposed to increased wall shear stress (after ligation of the left common carotid artery) were stained for hsp 60. The vessel wall of the left low-shear-stress-exposed side was negative. These findings demonstrate that shear stress results in hsp 60 induction in endothelial cells in vivo and in vitro, providing the prerequisite for humoral and cellular reactions to endothelial hsp in the earliest stages of atherosclerosis.
- Subjects :
- Animals
Arteriosclerosis genetics
Autoantigens genetics
Blood Pressure
Blotting, Northern
Carotid Artery, Common cytology
Carotid Artery, Common physiopathology
Cells, Cultured
Chaperonin 60 analysis
Culture Media pharmacology
Endothelium, Vascular chemistry
Endothelium, Vascular cytology
Female
Gene Expression immunology
Humans
Ligation
Perfusion
RNA, Messenger analysis
Rats
Rats, Inbred Lew
Staining and Labeling
Stress, Mechanical
Umbilical Veins cytology
Viscosity
Arteriosclerosis immunology
Chaperonin 60 genetics
Chaperonin 60 immunology
Endothelium, Vascular immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1079-5642
- Volume :
- 20
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Arteriosclerosis, thrombosis, and vascular biology
- Publication Type :
- Academic Journal
- Accession number :
- 10712382
- Full Text :
- https://doi.org/10.1161/01.atv.20.3.617