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Suppressive effects of swainsonine and N-butyldeoxynojirimycin on human bone marrow neutrophil maturation.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2000 Mar 05; Vol. 269 (1), pp. 219-25. - Publication Year :
- 2000
-
Abstract
- The effects of the N-linked oligosaccharide inhibitors swainsonine and N-butyldeoxynojirimycin (NB-DNJ) on granulopoiesis was investigated using human bone marrow cells in in vitro liquid and agar cultures. The addition of the inhibitors into cultures containing granulocyte colony-stimulating factor (G-CSF) suppressed maturation from myelocytes into mature neutrophils. Swainsonine did not induce apoptosis, but NB-DNJ induced considerable apoptosis, especially in the presence of G-CSF. This result indicated that the decrease of mature neutrophils by swainsonine was not because of cell degeneration. In the case of NB-DNJ, it was thought to be because of both maturation suppression and apoptosis. In a colony-forming unit-granuloid (CFU-G) colony assay, the number of colonies was increased in the presence of the inhibitors, but the morphology of colonies was predominantly compact, or immature. The inhibitors also suppressed the expressions of mRNAs of CCAAT/enhancer binding protein epsilon (C/EBPepsilon) and G-CSF receptor as markers of terminal neutrophil maturation. These findings suggested that the incompleteness of N-linked oligosaccharide leads to the suppression of terminal neutrophil maturation.<br /> (Copyright 2000 Academic Press.)
- Subjects :
- 1-Deoxynojirimycin pharmacology
Apoptosis drug effects
Base Sequence
Bone Marrow Cells metabolism
CCAAT-Enhancer-Binding Proteins
Cell Differentiation drug effects
Cells, Cultured
Colony-Forming Units Assay
DNA Primers genetics
DNA-Binding Proteins genetics
Enzyme Inhibitors pharmacology
Glycoside Hydrolase Inhibitors
Granulocyte Colony-Stimulating Factor pharmacology
Hematopoiesis drug effects
Humans
Mannosidases antagonists & inhibitors
Neutrophils metabolism
Nuclear Proteins genetics
Oligosaccharides metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Receptors, Granulocyte Colony-Stimulating Factor genetics
Tunicamycin pharmacology
alpha-Mannosidase
1-Deoxynojirimycin analogs & derivatives
Bone Marrow Cells cytology
Bone Marrow Cells drug effects
Neutrophils cytology
Neutrophils drug effects
Swainsonine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 269
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 10694503
- Full Text :
- https://doi.org/10.1006/bbrc.2000.2269