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Region-specific changes in 5-HT(1A) receptor-activated G-proteins in rat brain following chronic buspirone.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2000 Feb 18; Vol. 389 (2-3), pp. 147-53. - Publication Year :
- 2000
-
Abstract
- 5-Hydroxytryptamine(1A) (5-HT(1A)) receptors, which activate inhibitory G-proteins, are implicated in psychiatric disorders including anxiety and depression. Studies suggest that chronic 5-HT(1A) receptor agonist administration alters 5-HT(1A) receptor function, but the effect of chronic treatment on 5-HT(1A) receptor-activated G-proteins is unclear. In this study, agonist-stimulated [35S]guanylyl-5'-O-(gamma-thio)-triphosphate (GTPgammaS) binding was examined following chronic administration of buspirone. Brains were processed for [35S]GTPgammaS autoradiography using R(+)-8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) for 5-HT(1A) receptors or baclofen for GABA(B) receptors. Net 8-OH-DPAT-stimulated [35S]GTPgammaS binding was decreased by 25-30% in the septum and dorsal raphe nucleus of buspirone-treated animals. No significant changes in 8-OH-DPAT-stimulated [35S]GTPgammaS binding were found in the prefrontal, entorhinal or cingulate cortices or hippocampus in buspirone-treated rats. GABA(B) receptor-stimulated [35S]GTPgammaS binding was increased by 25% in the hippocampus, with no significant changes in any other region examined. These results demonstrate region-specific alterations in 5-HT(1A) and GABA(B) receptor-activated G-proteins following chronic buspirone treatment, which may contribute to the clinical effects of this drug.
- Subjects :
- 8-Hydroxy-2-(di-n-propylamino)tetralin pharmacology
Animals
Guanosine 5'-O-(3-Thiotriphosphate) metabolism
Male
Rats
Rats, Sprague-Dawley
Receptors, GABA-B physiology
Receptors, Serotonin drug effects
Receptors, Serotonin, 5-HT1
Brain Chemistry drug effects
Buspirone pharmacology
GTP-Binding Proteins analysis
Receptors, Serotonin physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2999
- Volume :
- 389
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 10688978
- Full Text :
- https://doi.org/10.1016/s0014-2999(99)00875-4