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Recombinant Semliki Forest virus particles expressing louping ill virus antigens induce a better protective response than plasmid-based DNA vaccines or an inactivated whole particle vaccine.
- Source :
-
The Journal of general virology [J Gen Virol] 2000 Mar; Vol. 81 (Pt 3), pp. 749-58. - Publication Year :
- 2000
-
Abstract
- Louping ill virus (LIV) infection of mice was used as a model to evaluate the protective efficacy of Semliki Forest virus (SFV)-based vaccines in comparison to a standard DNA vaccine and a commercial chemically inactivated vaccine. The recombinant SFV-based vaccines consisted of suicidal particles and a naked layered DNA/RNA construct. The nucleic acid vaccines expressed the spike precursor prME and the nonstructural protein 1 (NS1) antigens of LIV. Three LIV strains of graded virulence for mice were used for challenge. One of these was a naturally occurring antibody escape variant. All vaccines tested induced humoral immunity but gave varying levels of protection against lethal challenge. Only recombinant SFV particles administered twice gave full protection against neuronal degeneration and encephalitis induced by two of the three challenge strains, and partial protection against the highly virulent strain, whereas the other vaccines tested gave lower levels of partial protection.
- Subjects :
- Animals
Antibodies, Viral biosynthesis
Cell Line
Cricetinae
Encephalitis Viruses, Tick-Borne pathogenicity
Encephalitis, Tick-Borne immunology
Encephalitis, Tick-Borne pathology
Encephalitis, Tick-Borne prevention & control
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Models, Biological
Plasmids genetics
Recombination, Genetic
Vaccines, DNA genetics
Vaccines, DNA immunology
Vaccines, Inactivated genetics
Vaccines, Inactivated immunology
Antigens, Viral genetics
Encephalitis Viruses, Tick-Borne genetics
Encephalitis Viruses, Tick-Borne immunology
Semliki forest virus genetics
Semliki forest virus immunology
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Viral Vaccines genetics
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 81
- Issue :
- Pt 3
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 10675413
- Full Text :
- https://doi.org/10.1099/0022-1317-81-3-749