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Assessment of a recombinant antigen versus natural hypodermin C for the serodiagnosis of hypodermosis in cattle.
- Source :
-
Parasitology research [Parasitol Res] 2000 Jan; Vol. 86 (1), pp. 67-8. - Publication Year :
- 2000
-
Abstract
- An indirect ELISA test using as antigen a recombinant parasite protein, hypodermin C, was developed to measure Hypoderma-specific antibodies in cattle sera and compared with natural hypodermin C. To evaluate the field efficacy of the ELISA test, 334 serum samples were collected from cows raised at farms in Galicia for a serological survey. Compared with an ELISA based on natural parasite antigen, the recombinant hypodermin C gave excellent results, with a sensitivity of 95.8% and a specificity of 95.7%. Considering the cut-off point, with the recombinant hypodermin C, 70.9% of the animals had positive levels of antibodies to Hypoderma and with natural hypodermin C, 73.6%. Recombinant hypodermin C appears to be a useful alternative to the natural parasite antigen for the serodiagnosis of Hypoderma sp in cattle.
- Subjects :
- Animals
Cattle
Cattle Diseases parasitology
Diptera immunology
Diptera metabolism
Enzyme-Linked Immunosorbent Assay
Hypodermyiasis diagnosis
Hypodermyiasis parasitology
Recombinant Proteins immunology
Serine Endopeptidases genetics
Antibodies blood
Antigens immunology
Cattle Diseases diagnosis
Hypodermyiasis veterinary
Serine Endopeptidases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0932-0113
- Volume :
- 86
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 10669139
- Full Text :
- https://doi.org/10.1007/s004360050012