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Crc is involved in catabolite repression control of the bkd operons of Pseudomonas putida and Pseudomonas aeruginosa.
- Source :
-
Journal of bacteriology [J Bacteriol] 2000 Feb; Vol. 182 (4), pp. 1144-9. - Publication Year :
- 2000
-
Abstract
- Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd operons of P. putida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated in both species by catabolite repression. We report here that this effect is mediated in both species by Crc. A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced. Crc regulates the expression of branched-chain keto acid dehydrogenase, glucose-6-phosphate dehydrogenase, and amidase in both species but not urocanase, although the carbon sources responsible for catabolite repression in the two species differ. Transposon mutants affected in their expression of BkdR, the transcriptional activator of the bkd operon, were isolated and identified as crc and vacB (rnr) mutants. These mutants suggested that catabolite repression in pseudomonads might, in part, involve control of BkdR levels.
- Subjects :
- 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Amidohydrolases genetics
Amidohydrolases metabolism
DNA Transposable Elements
DNA-Binding Proteins metabolism
Ketone Oxidoreductases metabolism
Leucine-Responsive Regulatory Protein
Molecular Sequence Data
Multienzyme Complexes metabolism
Mutagenesis
Plasmids genetics
Pseudomonas genetics
Pseudomonas aeruginosa enzymology
Pseudomonas aeruginosa genetics
Pseudomonas putida enzymology
Pseudomonas putida genetics
Recombination, Genetic
Repressor Proteins isolation & purification
Repressor Proteins metabolism
Ribonucleases genetics
Ribonucleases metabolism
Sequence Analysis, DNA
Urocanate Hydratase genetics
Urocanate Hydratase metabolism
Bacterial Proteins
DNA-Binding Proteins genetics
Gene Expression Regulation, Bacterial
Ketone Oxidoreductases genetics
Multienzyme Complexes genetics
Operon
Pseudomonas enzymology
Repressor Proteins genetics
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 182
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 10648542
- Full Text :
- https://doi.org/10.1128/JB.182.4.1144-1149.2000