Back to Search
Start Over
Virulent or avirulent (dhfr-ts-) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro.
- Source :
-
Clinical and experimental immunology [Clin Exp Immunol] 2000 Feb; Vol. 119 (2), pp. 299-304. - Publication Year :
- 2000
-
Abstract
- In this study we have compared the immune response of normal human cells cultured in vitro to two virulent strains of Leishmania major (CC1 and LV39), and to an avirulent vaccine strain (dhfr-ts-) made by targeted deletion of the essential gene DHFR-TS. We utilized an in vitro system in which naive T cells from normal human donors were primed with autologous Leishmania-infected macrophages. All three parasites infected macrophages and transformed into amastigotes within the cells. However, whereas LV39 and CC1 replicated in macrophages, dhfr-ts- did not. When peripheral blood lymphocytes (PBL) were stimulated with autologous macrophages infected with any of the three parasites, the lymphocytes produced a type-1-biased cytokine response. Finally, addition of IL-12 during the first stimulation period increased the production of interferon-gamma but decreased IL-5 secretion. On the other hand, anti-IL-12 resulted in the opposite effect.
- Subjects :
- Animals
Cells, Cultured
Humans
Interferon-gamma biosynthesis
Interleukin-12 physiology
Interleukin-5 biosynthesis
Leishmania major enzymology
Leishmania major genetics
Tetrahydrofolate Dehydrogenase deficiency
Tetrahydrofolate Dehydrogenase genetics
Th1 Cells metabolism
Th1 Cells parasitology
Th2 Cells immunology
Th2 Cells metabolism
Th2 Cells parasitology
Thymidylate Synthase deficiency
Thymidylate Synthase genetics
Virulence
Cytokines biosynthesis
Leishmania major immunology
Leishmania major pathogenicity
Th1 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9104
- Volume :
- 119
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinical and experimental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 10632666
- Full Text :
- https://doi.org/10.1046/j.1365-2249.2000.01122.x