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In vitro erythroid colony formation in acute myelogenous leukemia in the rat.

Authors :
Handler ES
Handler EE
Source :
Journal of the National Cancer Institute [J Natl Cancer Inst] 1976 Apr; Vol. 56 (4), pp. 851-3.
Publication Year :
1976

Abstract

Erythroid colonies were grown in vitro in plasma clot cultures. Normal adult rat bone marrow responded to exogenous erythropoietin with the formation of an average of 2 colonies/10(3) cells plated. No erythroid colonies were observed in cultured normal spleen preparations. Shay chloro-leukemia cells administered iv induced an acute myelogenous leukemia. During the progressive stages of the disease, the numbers of erythrocyte colony forming units (CFU-E) in the marrow decreased; concomitantly, these progenitors appeared in leukemic spleen cultures. Paralleling changes in CFU-E, the numbers of nucleated red blood cells in the marrow declined but increased in the leukemic spleen. However, compensatory spleen erythropoiesis was transient, due to continued leukemia cell colonization. The loss of erythroid progenitor cells from the bone marrow played a significant role in the anemia associated with this leukemia.

Details

Language :
English
ISSN :
0027-8874
Volume :
56
Issue :
4
Database :
MEDLINE
Journal :
Journal of the National Cancer Institute
Publication Type :
Academic Journal
Accession number :
1062627
Full Text :
https://doi.org/10.1093/jnci/56.4.851