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Detection of pseudorabies virus DNA in individual single-reactor pigs found in certified pseudorabies-free herds.
- Source :
-
Research in veterinary science [Res Vet Sci] 1999 Dec; Vol. 67 (3), pp. 305-7. - Publication Year :
- 1999
-
Abstract
- During monitoring of certified pseudorabies (PRV)-free herds to confirm their PRV -free status, occasional individual gE-seropositive pigs are detected. These single-reactor pigs remain gE-seropositive when further serum samples are collected and tested. For the eradication programme to proceed, it is important to determine whether these pigs are only false positives or are; in fact, infected with field PRV. The purpose of this study was to determine whether the polymerase chain reaction (PCR) could detect field PRVDNA in single-reactor pigs and so confirm positive reactions in the serologic monitoring programme. First, DNA samples of various tissues from 15 single-reactor pigs all from different herds were examined for field PRV by PCR. Additionally, serum samples from these pigs were analyzed in a gE-confirmation enzyme linked immunosorbent assay (gE-confirmation ELISA). PCR detected PRVDNA in five of the 15 pigs, and these results were confirmed by the gE-confirmation ELISA. The remaining 10 pigs that tested negative in the PCR also tested negative in the gE- confirmation ELISA. We conclude that PCR can be used to discriminate between true and false serological positive single-reactor pigs and, moreover, that the gE-confirmation ELISA confirms these PCR results.<br /> (Copyright 1999 Harcourt Publishers Limited.)
Details
- Language :
- English
- ISSN :
- 0034-5288
- Volume :
- 67
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Research in veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 10607513
- Full Text :
- https://doi.org/10.1053/rvsc.1999.0316