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Assignment of cytosine N3 resonances in nucleic acids via intrabase three-bond coupling to amino protons.
- Source :
-
Journal of biomolecular NMR [J Biomol NMR] 1999 Oct; Vol. 15 (2), pp. 173-6. - Publication Year :
- 1999
-
Abstract
- Coherences were observed between 15N3 of cytosine and its trans amino proton (H42) using a modified gradient-based heteronuclear single quantum coherence (HSQC) pulse sequence optimized for three-bond proton-nitrogen couplings. The method is demonstrated with a 22-nucleotide RNA fragment of the P5abc region of a group I intron uniformly labeled with 15N. Use of intraresidue 15N3-amino proton couplings to assign cytosine 15N3 signals complements the recently proposed JNN HNN COSY [Dingley, A.J. and Grzesiek, S. (1998) J. Am. Chem. Soc., 120, 8293-8297] method of identifying hydrogen-bonded base pairs in RNA.
Details
- Language :
- English
- ISSN :
- 0925-2738
- Volume :
- 15
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of biomolecular NMR
- Publication Type :
- Academic Journal
- Accession number :
- 10605090
- Full Text :
- https://doi.org/10.1023/a:1008359723515