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Detection of methylation damage in DNA of gastric cancer tissues using 32P postlabelling assay.
- Source :
-
Japanese journal of cancer research : Gann [Jpn J Cancer Res] 1999 Oct; Vol. 90 (10), pp. 1104-8. - Publication Year :
- 1999
-
Abstract
- Gastric cancer is the most common cancer in Korea. The causes are still unknown but it has been speculated that gastric cancer is associated with consumption of foods rich in nitrates/nitrites or a high dietary intake of salt or pickled food. In the present study, we studied the level of alkylated DNA adducts formed in gastric cancer tissues in comparison with that in normal gastric mucosa. DNA was extracted from surgically removed gastric cancer tissues and patient-matched normal gastric mucosa. The level of N7-methyldeoxyguanosine was measured by 32P-postlabelling assay after high performance liquid chromatography (HPLC) enrichment. We found that the level of N7-methyldeoxyguanosine of gastric cancerous tissues was significantly higher than that of normal gastric mucosa (P=0.01685).
- Subjects :
- Adenocarcinoma pathology
Adenocarcinoma surgery
Chromatography, High Pressure Liquid
DNA Adducts isolation & purification
DNA, Neoplasm metabolism
Deoxyguanosine analogs & derivatives
Deoxyguanosine analysis
Humans
Phosphorus Radioisotopes
Radioisotope Dilution Technique
Stomach Neoplasms pathology
Stomach Neoplasms surgery
Adenocarcinoma genetics
DNA Damage
DNA Methylation
DNA, Neoplasm genetics
Stomach Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0910-5050
- Volume :
- 90
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Japanese journal of cancer research : Gann
- Publication Type :
- Academic Journal
- Accession number :
- 10595739
- Full Text :
- https://doi.org/10.1111/j.1349-7006.1999.tb00684.x