Back to Search
Start Over
Spatiotemporal changes of fibronectin, tenascin-C, fibulin-1, and fibulin-2 in the skin during the development of chronic contact dermatitis.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 1999 Dec; Vol. 113 (6), pp. 906-12. - Publication Year :
- 1999
-
Abstract
- In order to elucidate how chronic inflammation affects the organization of the extracellular matrix in the skin, a prolonged allergic contact dermatitis was induced in a mouse by repeated application to the ear of 2,4-dinitrofluorobenzene every 3 d for 66 d. Subsequently, the spatiotemporal changes of fibronectin, tenascin-C, fibulin-1, and fibulin-2 in the skin were examined. In the acute phase of inflammation (day 3-day 12), the amount of fibronectin and tenascin-C increased markedly and were degraded, whereas the amount of fibulin-2 changed slightly. Abundant deposition of tenascin-C was observed in the connective tissue. Fibulin-1 and fibulin-2 distributed as fine fibrils. In contrast, the amounts of fibronectin and tenascin-C decreased and their degradation was suppressed in the chronic phase (day 15-day 66), but the amount of fibulin-2 increased. Tenascin-C was observed mainly at and underneath the epidermal basement membrane. In the subepidermal region, many fibulin-2-positive microfibrils were distributed. The amount and distribution of fibulin-1 did not change markedly in either phase. MMP-like enzymes of 62 kDa, probably activated MMP-2, were upregulated in the chronic phase, whereas components of 92, 85, or 67 kDa were highly induced in the acute phase. These results suggest that chronic inflammation in allergic contact dermatitis is associated with temporal changes in the expression, deposition, and degradation of inducible extracellular matrix components.
- Subjects :
- Animals
Blotting, Western
Chronic Disease
Dermatitis, Contact pathology
Female
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 metabolism
Mice
Mice, Inbred BALB C
T-Lymphocyte Subsets immunology
Calcium-Binding Proteins analysis
Dermatitis, Contact metabolism
Extracellular Matrix Proteins analysis
Fibronectins analysis
Skin chemistry
Tenascin analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-202X
- Volume :
- 113
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 10594729
- Full Text :
- https://doi.org/10.1046/j.1523-1747.1999.00802.x