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Resolution of high-molecular-weight components in lipopolysaccharides of Escherichia coli, Morganella morganii, Citrobacter freundii and Citrobacter diversus strains with sodium dodecyl sulfate polyacrylamide gels.

Authors :
Tavío MM
Vila J
Ruiz J
Ruiz J
Martín-Sánchez AM
Jiménez de Anta MT
Source :
Journal of microbiological methods [J Microbiol Methods] 2000 Jan; Vol. 39 (2), pp. 145-8.
Publication Year :
2000

Abstract

The use of 0.5% sodium dodecyl sulfate in polyacrylamide separation gels allowed the resolution in several bands of high-molecular-mass components in smooth lipopolysaccharide of bacterial outer membrane from Escherichia coli, Morganella morganii, Citrobacter freundii and Citrobacter diversus. With or without 0.1% SDS, however, such a result was not possible.

Details

Language :
English
ISSN :
0167-7012
Volume :
39
Issue :
2
Database :
MEDLINE
Journal :
Journal of microbiological methods
Publication Type :
Academic Journal
Accession number :
10576704
Full Text :
https://doi.org/10.1016/s0167-7012(99)00109-8