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Rab5 regulates motility of early endosomes on microtubules.
- Source :
-
Nature cell biology [Nat Cell Biol] 1999 Oct; Vol. 1 (6), pp. 376-82. - Publication Year :
- 1999
-
Abstract
- The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.
- Subjects :
- Biological Transport
Cell Line
Green Fluorescent Proteins
HeLa Cells
Humans
Luminescent Proteins genetics
Luminescent Proteins metabolism
Phosphatidylinositol 3-Kinases metabolism
Recombinant Fusion Proteins genetics
rab5 GTP-Binding Proteins genetics
Endocytosis physiology
Endosomes physiology
Microtubules physiology
rab5 GTP-Binding Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1465-7392
- Volume :
- 1
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Nature cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 10559966
- Full Text :
- https://doi.org/10.1038/14075