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Improved detection of enterotoxigenic Escherichia coli among patients with travelers' diarrhea, by use of the polymerase chain reaction technique.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 1999 Dec; Vol. 180 (6), pp. 2053-5. - Publication Year :
- 1999
-
Abstract
- This study sought to determine whether a specific polymerase chain reaction (PCR) for enterotoxigenic Escherichia coli (ETEC) toxins after chaotropic extraction of DNA from stool would increase the detection of ETEC over that of conventional oligonucleotide probe hybridization of 5 E. coli colonies per stool sample (a standard method). By DNA hybridization, 29 (21%) of 140 patients were positive for ETEC, and 59 (42%) of 140 were positive for ETEC when PCR was used. Sensitivity of the PCR assay was confirmed through spiked stool experiments to be approximately 100-1000 ETEC colonies per sample. Specificity of the assay was determined by showing an absence of ETEC by the PCR technique in a subgroup of 48 subjects and by confirming the presence of ETEC DNA of positive samples by dot blot procedure. PCR technique detected significantly more ETEC infections in these subjects than did the hybridization method (P<.0001).
- Subjects :
- Bacterial Toxins biosynthesis
Bacterial Toxins genetics
DNA, Bacterial analysis
DNA, Bacterial isolation & purification
Diarrhea diagnosis
Enterotoxins biosynthesis
Enterotoxins genetics
Escherichia coli metabolism
Escherichia coli Infections microbiology
Feces microbiology
Humans
Nucleic Acid Hybridization
Sensitivity and Specificity
Travel
Bacterial Toxins analysis
Diarrhea microbiology
Enterotoxins analysis
Escherichia coli isolation & purification
Escherichia coli Infections diagnosis
Escherichia coli Proteins
Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 180
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 10558969
- Full Text :
- https://doi.org/10.1086/315121