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Evaluation of distinct blood lymphocyte populations in human immunodeficiency virus type 1-infected subjects in the absence or presence of effective therapy.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 1999 Dec; Vol. 180 (6), pp. 1851-62. - Publication Year :
- 1999
-
Abstract
- Virus reservoirs can persist in human immunodeficiency virus type 1 (HIV-1)-infected subjects despite effective plasma virus suppression. To compare viral dynamics in the absence and presence of antiretroviral therapy, blood mononuclear cells from 19 subjects with high plasma RNA levels and 18 subjects following prolonged virus suppression were examined, by use of in situ hybridization, to detect virus RNA expression before and after in vitro T cell activation. This approach reveals circulating lymphocytes expressing HIV-1 RNA before activation and an increase in cells with detectable HIV-1 RNA transcription after in vitro activation. The frequencies of these 2 cell populations are strongly correlated with plasma virus load and appear to be stable once a new steady state is established during therapy. The frequency of viral RNA-positive cells is equivalent to the frequency of cells that produce infectious virus. Thus, in HIV-1-infected subjects there are distinct virus reservoirs comprising both latent and replication-active cells.
- Subjects :
- Cells, Cultured
DNA, Viral blood
Drug Therapy, Combination
Freezing
HIV-1 genetics
Humans
In Situ Hybridization
Lymphocyte Activation
Proviruses
RNA, Viral blood
RNA, Viral genetics
T-Lymphocytes immunology
Viral Load
Virus Activation
Virus Latency
Anti-HIV Agents therapeutic use
HIV Infections drug therapy
HIV Infections virology
HIV-1 physiology
Leukocytes, Mononuclear virology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 180
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 10558941
- Full Text :
- https://doi.org/10.1086/315117