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A rat small bowel transplant model of chronic rejection: histopathologic characteristics.
- Source :
-
Transplantation [Transplantation] 1999 Sep 27; Vol. 68 (6), pp. 766-79. - Publication Year :
- 1999
-
Abstract
- Background: The major impediment to long-term success in solid organ transplantation is the development of chronic rejection (CR). The vascular lesion of CR, transplant vascular sclerosis (TVS) is characterized by neointimal smooth muscle cell proliferation, and is driven by both immune- and nonimmune-mediated mechanisms. Although the features of chronic heart and kidney allograft rejection have been well characterized, the more immunogenic small bowel allograft has not received similar study.<br />Methods: F344 small bowel (SB) was transplanted heterotopically into Lewis recipients that were treated with low-dose Cyclosporine A for 15 days. Lewis recipients of F344 or Lewis SB grafts without immunosuppression, served as controls. Grafts were assessed histologically when recipients showed clinical signs of rejection or at predetermined time points. The immunological components involved in the chronic rejection process were evaluated by immunohistochemical staining.<br />Results: All SB allografts (100%) developed histologic evidence of CR Cyclosporine A. TVS was seen in 36 of the 46 (78%) of these allografts. The median time to develop TVS was 45 days. Immunohistochemical staining of chronically rejected grafts showed infiltration predominantly by CD4+ cells and macrophages, uniform up-regulation of class II MHC molecule expression, moderate to intense ICAM-1 staining in grafts harvested at postoperative day 45, and uniform neointimal cell staining for smooth muscle cell alpha-actin in the TVS lesions.<br />Conclusions: This F344 to Lewis SB transplant model is a useful model that reproduces significant features of CR. The highly immunogenic nature of the SB allografts allows this model to serve as a stringent test for protocols designed to prevent CR.
- Subjects :
- Animals
Arteriosclerosis etiology
CD4-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Cyclosporine pharmacology
Disease Models, Animal
Fibrosis
Graft Rejection complications
Graft Rejection pathology
Graft Survival drug effects
Graft Survival physiology
Histocompatibility Antigens Class II immunology
Immunohistochemistry
Intestine, Small pathology
Macrophages immunology
Male
Mesenteric Vascular Occlusion etiology
Mesentery pathology
Muscle, Smooth, Vascular cytology
Rats
Rats, Inbred F344
Rats, Inbred Lew
Time Factors
Intestine, Small transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 0041-1337
- Volume :
- 68
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 10515376
- Full Text :
- https://doi.org/10.1097/00007890-199909270-00008