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Nitric oxide donors stimulate prostaglandin F(2alpha) and inhibit thromboxane B(2) production in the human cervix during the first trimester of pregnancy.
- Source :
-
Molecular human reproduction [Mol Hum Reprod] 1999 Oct; Vol. 5 (10), pp. 973-82. - Publication Year :
- 1999
-
Abstract
- Nitric oxide (NO) donors are capable of ripening the human cervix during pregnancy. The purpose of this study was to examine how NO donors may be involved in this process. Cervical biopsies were obtained from pregnant women randomized to receive isosorbide mononitrate (n = 10) or no treatment (n = 10) prior to suction termination. Enzyme-linked immunosorbent assays (ELISA) were performed on culture supernatant for interleukin (IL)-1, IL-6, IL-8, IL-10, IL-15, tumour necrosis factor-alpha, monocyte chemoattractant protein-1 and prostaglandin metabolites. Immunohistochemistry was performed to localize these cytokines, cyclooxygenase (COX)-1, COX-2 and prostaglandin dehydrogenase in cervical tissue and reverse transcription-polymerase chain reaction (RT-PCR) to identify COX-1 and COX-2 expression. Biopsies treated with the NO donor isosorbide mononitrate (IMN) produced significantly greater amounts of prostaglandin F(2alpha) in culture and lower amounts of thromboxane B(2) than controls (572.8 versus 34.9 pg/ml, P < 0.05; 53.3 pg/ml versus 530.9 pg/ml, P < 0.01 respectively). The release of other prostaglandins and of cytokines was not affected by treatment with NO. Inflammatory mediators were localized to cervical tissue and COX-1 and COX-2 expression was confirmed by RT-PCR. In conclusion, the mechanism of NO donor-induced cervical ripening during pregnancy may be mediated in part via increased prostaglandin F(2alpha) synthesis.
- Subjects :
- Base Sequence
Cervical Ripening drug effects
Cervical Ripening physiology
Cervix Uteri immunology
Cyclooxygenase 1
Cyclooxygenase 2
Cytokines metabolism
DNA Primers genetics
Female
Humans
Immunohistochemistry
Isoenzymes genetics
Isosorbide Dinitrate pharmacology
Membrane Proteins
Pregnancy
Pregnancy Trimester, First
Prostaglandin-Endoperoxide Synthases genetics
Prostaglandins metabolism
Reverse Transcriptase Polymerase Chain Reaction
Cervix Uteri drug effects
Cervix Uteri metabolism
Dinoprost biosynthesis
Isosorbide Dinitrate analogs & derivatives
Nitric Oxide Donors pharmacology
Thromboxane B2 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1360-9947
- Volume :
- 5
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecular human reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 10508227
- Full Text :
- https://doi.org/10.1093/molehr/5.10.973