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Enterotrophic effects of glucagon-like peptide 2 are enhanced by neurotensin.
- Source :
-
Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract [J Gastrointest Surg] 1999 Jul-Aug; Vol. 3 (4), pp. 432-39; discussion 439-40. - Publication Year :
- 1999
-
Abstract
- Combination therapy with enterotrophic agents may be useful in patients with the short bowel syndrome. The gut hormones neurotensin (NT) and glucagon-like peptide 2 (GLP-2) are potent enterotrophic factors when administered alone; however, their combined effects are not known. Using a GLP-2-producing tumor (STC-1), we determined whether administration of NT enhances the effect of GLP-2 on intestinal growth. Athymic mice were injected with STC-1 cells (6 x 10(6)) subcutaneously. Twenty-three days after STC-1 implantation, mice received either NT (300 microg/kg or 600 microg/kg) or saline solution (control) subcutaneously three times a day for 6 days. Two groups of tumor-free mice received either saline or NT for 6 days. At sacrifice, jejunum and ileum were collected, weighed, and analyzed for DNA and protein content. In the jejunum, NT combined with GLP-2 (from STC-1) increased weight, protein content (markers of mucosal hypertrophy), and DNA content (a marker of mucosal hyperplasia), compared to either NT or GLP-2 alone. In the ileum, the combination of NT and GLP-2 significantly increased weight and/or protein content compared to NT or GLP-2 alone. Administration of NT enhances the enterotrophic effects of GLP-2, augmenting hypertrophy of the entire small bowel and hyperplasia of the jejunum. The combination of NT and GLP-2 may be useful to enhance intestinal growth in patients with the short bowel syndrome.
- Subjects :
- Analysis of Variance
Animals
DNA analysis
Drug Synergism
Gastrointestinal Hormones administration & dosage
Glucagon administration & dosage
Glucagon immunology
Glucagon-Like Peptide 2
Glucagon-Like Peptides
Glucagonoma pathology
Hyperplasia
Hypertrophy
Ileum chemistry
Ileum growth & development
Injections, Subcutaneous
Intestinal Mucosa drug effects
Intestinal Mucosa growth & development
Intestinal Mucosa pathology
Jejunum chemistry
Jejunum growth & development
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Neurotensin administration & dosage
Organ Size
Peptides administration & dosage
Protein Precursors administration & dosage
Proteins analysis
Short Bowel Syndrome drug therapy
Tumor Cells, Cultured
Gastrointestinal Hormones therapeutic use
Glucagon therapeutic use
Ileum drug effects
Jejunum drug effects
Neurotensin therapeutic use
Peptides therapeutic use
Protein Precursors therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1091-255X
- Volume :
- 3
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
- Publication Type :
- Academic Journal
- Accession number :
- 10482697
- Full Text :
- https://doi.org/10.1016/s1091-255x(99)80061-4