Back to Search
Start Over
The human B cell response to IL-13 is dependent on cellular phenotype as well as mode of activation.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1999 Sep 15; Vol. 163 (6), pp. 3185-93. - Publication Year :
- 1999
-
Abstract
- Normal mature quiescent human B lymphocytes, isolated as a function of buoyant density, require activation for up-regulation of IL-13R constituents. Cell activation through a combination of surface Ig and CD40 receptor ligation leads to the most substantial message production for IL-13Ralpha1. Functional consequences of this receptor variation, in initially quiescent cells, includes demonstrable effects on cellular proliferation in response to ligand exposure. Variations in the method of surface activation, with particular emphasis on the CD40 receptor, reveals that immobilized CD40 ligand may be sufficient, in and of itself, to up-regulate IL-13Ralpha1, which may bear significance for B-lymphocyte bystander proliferation. Regulation of the IL-13Ralpha1 protein and message also differs as a function of cellular phenotype. Although values are greater in memory than naive B cells, as they are initially isolated from extirpated tonsils, variations in the magnitude of message and protein, as a function of surface stimulation, are more substantial in the naive subset. The magnitude of variation in message production in naive cells is associated with a more vigorous proliferative response to IL-13 than seen in memory lymphocytes. The cellular response to IL-13, as a function of activation and phenotype, is the converse of that demonstrated for IL-2. Evaluation of proliferation, receptor message, ligand binding protein production, and the response to putatively synergistic cytokines reveals that IL-2 is the predominant lymphokine utilized by memory cells. This is in contradistinction to IL-13, which along with IL-4, are the predominant moieties for naive lymphocytes.
- Subjects :
- B-Lymphocyte Subsets cytology
CD40 Antigens metabolism
CD40 Antigens physiology
CD40 Ligand
Cell Division genetics
Cell Division immunology
Cells, Cultured
Cytokines physiology
Humans
Immunologic Memory immunology
Interleukin-13 metabolism
Interleukin-13 Receptor alpha1 Subunit
Interphase immunology
Ligands
Lymphocyte Activation genetics
Membrane Glycoproteins physiology
Palatine Tonsil
RNA, Messenger biosynthesis
Receptors, Interleukin biosynthesis
Receptors, Interleukin genetics
Receptors, Interleukin-13
Receptors, Interleukin-2 biosynthesis
Receptors, Interleukin-2 genetics
Receptors, Interleukin-4 biosynthesis
Receptors, Interleukin-4 genetics
B-Lymphocyte Subsets immunology
B-Lymphocyte Subsets metabolism
Immunophenotyping
Interleukin-13 physiology
Lymphocyte Activation immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 163
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 10477586