Back to Search
Start Over
Genomic organization of the S locus: Identification and characterization of genes in SLG/SRK region of S(9) haplotype of Brassica campestris (syn. rapa).
- Source :
-
Genetics [Genetics] 1999 Sep; Vol. 153 (1), pp. 391-400. - Publication Year :
- 1999
-
Abstract
- In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S(9) haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG(9), SRK(9), SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG(9). Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.
- Subjects :
- Amino Acid Sequence
Base Composition
Brassica enzymology
Cloning, Molecular
DNA Transposable Elements genetics
Gene Expression
Gene Expression Regulation, Plant
Gene Library
Genetic Linkage genetics
Genome, Plant
Molecular Sequence Data
Open Reading Frames genetics
Physical Chromosome Mapping
Plant Structures enzymology
Plant Structures genetics
Plant Structures growth & development
Polymorphism, Genetic genetics
RNA, Messenger analysis
RNA, Messenger genetics
Sequence Homology, Amino Acid
Brassica genetics
Genes, Plant genetics
Glycoproteins genetics
Haplotypes genetics
Plant Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0016-6731
- Volume :
- 153
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 10471721
- Full Text :
- https://doi.org/10.1093/genetics/153.1.391