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A PCR based DNA hybridisation capture system for the detection of human cytomegalovirus. A comparative study with other identification methods.
- Source :
-
Journal of virological methods [J Virol Methods] 1999 Jul; Vol. 80 (2), pp. 113-22. - Publication Year :
- 1999
-
Abstract
- A simple, sensitive and specific colourimetric hybridisation method for the detection of HCMV DNA in clinical specimens is described. This method combines a PCR assay with a sensitive sandwich hybridisation assay. It relies on the use of a specific capture probe linked covalently to polystyrene microplates and a specific polybiotinylated detection probe. Amplified DNA fragments, sandwiched between these two probes, are detected by an enzymatic colour reaction. This PCR-based colourimetric hybridisation method was compared with other known HCMV detection methods. Clinical specimens (n = 145, corresponding to 106 patients) were tested by both a nested PCR assay and this colourimetric hybridisation method; and by either the culture method or the pp65 antigenaemia test depending on the type of sample used. The results showed that the PCR-based hybridisation method has a specificity similar to tissue culture, known as the conventional gold standard method, and could be used for the examination of the clinical specimens.
- Subjects :
- Adolescent
Adult
Aged
Amniotic Fluid chemistry
Amniotic Fluid virology
Child
Child, Preschool
DNA, Viral blood
DNA, Viral cerebrospinal fluid
DNA, Viral urine
Endopeptidase K blood
Female
Humans
Male
Middle Aged
Placenta chemistry
Sensitivity and Specificity
Vitreous Body chemistry
Cytomegalovirus genetics
DNA, Viral isolation & purification
Nucleic Acid Hybridization methods
Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 0166-0934
- Volume :
- 80
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 10471020
- Full Text :
- https://doi.org/10.1016/s0166-0934(99)00023-3