Back to Search
Start Over
Nitric oxide synthase imunolabeling in the molluscan CNS and peripheral tissues.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1999 Aug 27; Vol. 262 (2), pp. 545-8. - Publication Year :
- 1999
-
Abstract
- NOS immunoreactivity was assayed in CNS and peripheral tissues of the sea slugs Pleurobranchaea californica, Tritonia diomedea and Aplysia californica using different antisera against mammalian nitric oxide synthase in Western blots. Polyclonal anti-nNOS labeled at 250, 185, 170, 155, 100, 75, and 65 kD in extracts of Pleurobranchaea CNS, salivary gland and esophagus but not of gills or muscle. The labeling pattern for Tritonia in bands at 250, 200, 120/110, 100, 69, 65, and 60 kD differed somewhat. Anti-nNOS labeling in Aplysia was markedly different, with bands labeled only at 69 and 60 kD in CNS extracts, and at 200, 190, 69 and 60 kD in salivary and esophagus extracts. The wide variation in NOS immunoreactivity is consistent with species differences in tissue localization and biochemical properties of molluscan NOS isoforms.<br /> (Copyright 1999 Academic Press.)
- Subjects :
- Animals
Aplysia enzymology
Blotting, Western
Humans
Isoenzymes immunology
Molecular Weight
Nitric Oxide Synthase immunology
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Rats
Species Specificity
Tissue Distribution
Isoenzymes isolation & purification
Mollusca enzymology
Nervous System enzymology
Nitric Oxide Synthase isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 262
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 10462511
- Full Text :
- https://doi.org/10.1006/bbrc.1999.1247