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The myoepithelial immunophenotype in 135 benign and malignant salivary gland tumors other than pleomorphic adenoma.
- Source :
-
Archives of pathology & laboratory medicine [Arch Pathol Lab Med] 1999 Sep; Vol. 123 (9), pp. 801-6. - Publication Year :
- 1999
-
Abstract
- Background: We have previously studied the immunoreactivity of 3 novel smooth muscle-specific proteins, alpha-smooth muscle actin, smooth muscle myosin heavy chains, and calponin, to assess myoepithelial differentiation in pleomorphic adenomas.<br />Objective: To further expand our knowledge of myoepithelial differentiation in other benign and malignant salivary gland tumors.<br />Design: Formalin-fixed paraffin sections of 135 salivary gland tumors with associated normal glands were stained with monoclonal antibodies using the avidin-biotin complex immunoperoxidase method and enzymatic and microwave heat-induced epitope retrieval.<br />Results: In adenoid cystic carcinomas and epithelial-myoepithelial carcinomas, all 3 markers exclusively highlighted the myoepithelial cell components and the epithelial cells were entirely negative. No immunostaining was detected in canalicular adenomas, oncocytomas, Warthin tumors, acinic cell carcinomas, mucoepidermoid carcinomas, squamous cell carcinomas, and polymorphous low-grade adenocarcinomas. Salivary duct carcinomas and adenocarcinomas, not otherwise specified had a distinctive pattern of uniform periductal staining of reactive myofibroblastic cells, and in salivary duct carcinomas some ducts retained a peripheral immunoreactive myoepithelial cell layer.<br />Conclusion: Immunoreactivity for these 3 smooth muscle-specific proteins confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas. Periductal linear staining in adenocarcinoma, not otherwise specified and salivary duct carcinomas is distinctive and appears to represent a tight cuff of myofibroblasts associated with the infiltrating glands.
- Subjects :
- Adenocarcinoma pathology
Adenolymphoma metabolism
Adenolymphoma pathology
Adenoma metabolism
Adenoma pathology
Adenoma, Oxyphilic metabolism
Adenoma, Oxyphilic pathology
Carcinoma, Acinar Cell metabolism
Carcinoma, Acinar Cell pathology
Carcinoma, Adenoid Cystic metabolism
Carcinoma, Adenoid Cystic pathology
Carcinoma, Mucoepidermoid metabolism
Carcinoma, Mucoepidermoid pathology
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Cell Differentiation
Humans
Immunohistochemistry
Microfilament Proteins
Muscle Proteins metabolism
Neoplasms pathology
Salivary Ducts metabolism
Salivary Ducts pathology
Salivary Gland Neoplasms pathology
Salivary Glands cytology
Salivary Glands metabolism
Calponins
Actins metabolism
Adenocarcinoma metabolism
Calcium-Binding Proteins metabolism
Myosin Heavy Chains metabolism
Neoplasms metabolism
Salivary Gland Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0003-9985
- Volume :
- 123
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Archives of pathology & laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 10458827
- Full Text :
- https://doi.org/10.5858/1999-123-0801-TMIIBA