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Reduction of IFN-gamma and IL-2 production by peripheral lymphocytes of HIV-exposed seronegative subjects.
- Source :
-
AIDS (London, England) [AIDS] 1999 Jul 30; Vol. 13 (11), pp. 1333-6. - Publication Year :
- 1999
-
Abstract
- Objectives: In order to evaluate the role played by cytokine profile as a co-factor involved in the resistance to HIV infection in couples serodiscordant for HIV, we studied HIV-seronegative subjects with multiple unprotected sexual exposures.<br />Design and Methods: Twenty-one HIV-exposed seronegative subjects (HEPS), their 21 HIV-seropositive partners and 10 HIV-seronegative unexposed individuals were studied for T helper (Th) types 1-2 cell pattern and CCR5 receptor.<br />Results: Twelve out of 21 HIV-seropositive partners of HEPS showed a CD4 cell count below 200 lymphocytes/microl. HIV strains were isolated from peripheral blood mononuclear cells (PBMC) in 17 patients (81%): seven subjects with syncytium-inducing strains and 10 with non-syncytium-inducing isolates. Low Th1 cytokine production and high levels of IL-4 were detected in HIV-seropositive subjects. A significant reduction of IL-2 and IFN-gamma expression in the CD4 and CD8 cells of HEPS was found compared with HIV-seronegative unexposed individuals. Similar levels of low IL-4 were present in both HEPS and controls. The partial deletion of a single allele (wild type/delta32) of CCR5 was found in only one HEPS.<br />Conclusion: The downregulated Th1 profile we observed in HEPS could be related to a cellular anergy state with a protective role in the transmission rate of HIV. Low levels of IL-2 and IFN-gamma could be involved in a low-grade activation state of CD4 lymphocytes. A decrease of IFN-gamma levels could render macrophage cells incapable of antigen presentation, thus resulting in a reduction of the cell-to-cell spread of infection.
- Subjects :
- CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes metabolism
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes metabolism
Cytokines biosynthesis
Female
HIV Seropositivity immunology
HIV Seropositivity virology
HIV-1 immunology
Humans
Male
RNA, Viral blood
Receptors, CCR5 genetics
Receptors, CCR5 metabolism
Sexual Behavior
T-Lymphocytes metabolism
HIV Seronegativity immunology
Interferon-gamma biosynthesis
Interleukin-2 biosynthesis
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0269-9370
- Volume :
- 13
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- AIDS (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 10449285
- Full Text :
- https://doi.org/10.1097/00002030-199907300-00009