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Differential inflammatory modulation of canine ileal longitudinal and circular muscle cells.
- Source :
-
The American journal of physiology [Am J Physiol] 1999 Aug; Vol. 277 (2), pp. G341-50. - Publication Year :
- 1999
-
Abstract
- The aim of this study was to identify the subtypes of muscarinic receptors that mediate in vivo and in vitro canine ileal longitudinal muscle contractions and whether their role is modulated by inflammation. Previous studies have reported that circular muscle contractions are suppressed in ileal inflammation induced by mucosal exposure to ethanol and acetic acid. We found that inflammation had no significant effect on in vivo and in vitro spontaneous or muscarinic receptor-mediated contractions of the longitudinal muscle. The longitudinal muscle contractions were mediated primarily by the M(3) receptor subtype. However, the IC(50) of the M(2) receptor antagonist methoctramine was only 10 times greater than that of the M(3) receptor antagonist 4-DAMP in the longitudinal muscle, whereas it was 224 times greater in the circular muscle. M(2) receptor-coupled decrease of intracellular cAMP occurred in the longitudinal but not in the circular muscle from the normal ileum. Inflammation did not alter this coupling in the longitudinal muscle but established it in the circular muscle. In conclusion, M(2) receptors may play a greater role in the mediation of longitudinal muscle contractions than circular muscle contractions. Inflammation does not alter the contractility or the relative role of muscarinic receptor subtypes in longitudinal muscle cells. However, it modulates the M(2) receptor coupling to adenylate cyclase in the circular muscle.
- Subjects :
- Adenylyl Cyclases metabolism
Animals
Dogs
Gastrointestinal Motility drug effects
Gastrointestinal Motility physiology
Ileitis pathology
Ileitis physiopathology
In Vitro Techniques
Injections, Intra-Arterial
Muscarine administration & dosage
Muscarine pharmacology
Muscle, Smooth drug effects
Muscle, Smooth pathology
Muscle, Smooth physiopathology
Protein Isoforms metabolism
Protein Isoforms physiology
Receptors, Muscarinic metabolism
Receptors, Muscarinic physiology
Ileitis metabolism
Muscle, Smooth metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 277
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 10444448
- Full Text :
- https://doi.org/10.1152/ajpgi.1999.277.2.G341